The subject matter of this work is the influence of e-business on customer relationships in this work we discuss the concept of e-business and its capabilities and principles of application or use. We have answered questions as what is e-business and what are the characteristics of e-business, and we compared the characteristics of e-business with characteristics of a traditional business. We have given some examples of companies using e-business and we sorted them by subjects that use e-business. In this paper we mentioned and briefly looked at his history of the Internet and the current situation of the Internet and we have determined its philosophy and standards. We gave an insight into the electronic business in Croatia since its first appearance until today. In customer relationships part of this work, we gave the answer on the importance of relations between the company and clients in e-business and we mentioned the development of the concept of managing the relationship between customers and company. We studied the importance of attracting clients and its impact on client’s decisions. In the practical part of this work, we took five retail chains that are operating on Croatian market and we analyzed their use of Web technology for the customer relationships. The analysis is based on Usability perspective; in this case, it is based on author’s perspective, who is also a customer of retail chains mentioned in the analysis. Retail chains that were selected for analysis are Billa, Spar, Kaufland, Lidl, and Konzum. This work has contributed on knowledge of e-business and its impact on the policy of customer relationships and it has contributed to the understanding of the purpose of using e-business to improve customer relationships and helped in the preparation of the analysis of companies web pages and ultimately on making the conclusion of this paper.